Author:
Xia Mengming,Xu Xinhao,Chen Chaojie,Wu Hualu,Xu Ren-ai,Wang Changlv
Abstract
AbstractIn this study, we firstly established and verified a method by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for the analysis of vilazodone and its metabolite M10 in rat plasma, then this method was used to explore the pharmacokinetics of vilazodone and M10 present or absence of 80 mg/kg bergenin in rats. Protein precipitation with acetonitrile was used to prepare the samples in this research. The mobile phase for liquid chromatography was consisted of 0.1% formic acid aqueous solution and acetonitrile. Brexpiprazole was used as the internal standard (IS), and the multiple reaction monitoring (MRM) mode was used for detection. The verification items required by the US Food and Drug Administration (FDA) guidelines such as selectivity, sensitivity, linearity, stability, recovery and matrix effect of this method were all met the standards. Besides, rats were used to explore the drug-drug interaction between vilazodone and bergenin, which were divided into two groups, and separately gavaged with the same-volume of carboxymethyl cellulose sodium (CMC-Na) solution and 80 mg/kg bergenin, respectively. The results showed that bergenin significantly affected the metabolism of vilazodone. It suggested that there was a potential drug-drug interaction between bergenin and vilazodone in rats. In clinical application, we should pay attention to the dose of vilazodone when in combination with bergenin.
Publisher
Springer Science and Business Media LLC
Reference23 articles.
1. Sun X, Huang W, Ma M, Guo B, Wang G. [Comparative studies on content of arbutin, bergenin and catechin in different part of Bergenia purpurascens and B. Crassifolia]. Zhongguo Zhong Yao Za Zhi. 2010;35(16):2079–82.
2. Koul B, Kumar A, Yadav D, Jin JO. Bergenia Genus: traditional uses, Phytochemistry and Pharmacology. Molecules 2020, 25(23).
3. Antimicrobial. Antiradical activity of extracts obtained from leaves of five species of the Genus Bergenia: identification of Antimicrobial compounds. Microb Drug Resist. 2017;23(6):771–80.
4. Koul B, Kumar A, Yadav D, Jin J-O. Bergenia Genus: traditional uses, Phytochemistry and Pharmacology. Molecules. 2020;25(23):5555.
5. Wang K, Li Y-f, Lv Q, Li X-m, Dai Y, Wei Z-f. Bergenin, acting as an agonist of PPARγ, ameliorates experimental colitis in mice through improving expression of SIRT1, and therefore inhibiting NF-κB-Mediated macrophage activation. Front Pharmacol 2018, 8.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献