Author:
Oskolkov Nikolay,Santel Malgorzata,Parikh Hemang M.,Ekström Ola,Camp Gray J.,Miyamoto-Mikami Eri,Ström Kristoffer,Mir Bilal Ahmad,Kryvokhyzha Dmytro,Lehtovirta Mikko,Kobayashi Hiroyuki,Kakigi Ryo,Naito Hisashi,Eriksson Karl-Fredrik,Nystedt Björn,Fuku Noriyuki,Treutlein Barbara,Pääbo Svante,Hansson Ola
Abstract
Abstract
Background
Skeletal muscle fiber type distribution has implications for human health, muscle function, and performance. This knowledge has been gathered using labor-intensive and costly methodology that limited these studies. Here, we present a method based on muscle tissue RNA sequencing data (totRNAseq) to estimate the distribution of skeletal muscle fiber types from frozen human samples, allowing for a larger number of individuals to be tested.
Methods
By using single-nuclei RNA sequencing (snRNAseq) data as a reference, cluster expression signatures were produced by averaging gene expression of cluster gene markers and then applying these to totRNAseq data and inferring muscle fiber nuclei type via linear matrix decomposition. This estimate was then compared with fiber type distribution measured by ATPase staining or myosin heavy chain protein isoform distribution of 62 muscle samples in two independent cohorts (n = 39 and 22).
Results
The correlation between the sequencing-based method and the other two were rATPas = 0.44 [0.13–0.67], [95% CI], and rmyosin = 0.83 [0.61–0.93], with p = 5.70 × 10–3 and 2.00 × 10–6, respectively. The deconvolution inference of fiber type composition was accurate even for very low totRNAseq sequencing depths, i.e., down to an average of ~ 10,000 paired-end reads.
Conclusions
This new method (https://github.com/OlaHanssonLab/PredictFiberType) consequently allows for measurement of fiber type distribution of a larger number of samples using totRNAseq in a cost and labor-efficient way. It is now feasible to study the association between fiber type distribution and e.g. health outcomes in large well-powered studies.
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Molecular Biology,Orthopedics and Sports Medicine
Cited by
5 articles.
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