Abstract
Abstract
Purpose
This study aimed to investigate changes in the bacterial diversity of the rhizosphere soil of slag mountains in different years of revegetation restoration.
Methods
Seven soil samples were selected from different years of revegetation restoration in Qinghai, China. The bacterial community of each soil was analyzed via high-throughput sequencing using the Illumina MiSeq platform.
Results
Statistical analyses revealed that the diversity of the soil bacterial community was higher in the soil that was restored in 2017 than that in the soils restored from other years. 16S rRNA sequencing revealed that Proteobacteria and Actinobacteria were the dominant phyla. Sphingomonas was the dominant genus. Total nitrogen, available nitrogen, and total potassium influenced the horizontal community structure of the phylum, whereas total nitrogen, organic matter, and pH had a great influence on the horizontal community structure of the phylum. The richness and diversity of the bacterial community in the soils that underwent revegetation restoration were greater in the third year (2017) than in other years. In the seventh year of recovery, the richness and diversity of the bacterial community began to decline.
Conclusion
The bacterial diversity of the soil in the coal mine slag mountain improved with the increase in vegetation restoration years.
Funder
the National Natural Science Foundation of China
Independent project of Ecosystem Succession and Management in the world Class Discipline of Ecology, Qinghai University
National Key Research and Development Plan Project
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology