Kadmiyuma (CD) maruz kalan dişi sıçanlarda Lactobacillus plantarum (LP) uygulamasının bazı kan parametreleri ve ANAE-pozitif lenfosit oranları üzerine kısmi koruyucu etkileri

Abstract

We aimed to research the influences of oral L.plantarum administration on some blood parameters and alpha-naphthyl acetate esterase (ANAE)-positive lymphocyte ratios in female rats exposed chronically to Cd. In order to perform this investigation, rats were separated into four equal groups as control (C, n = 8), cadmium (Cd, n = 8), L.plantarum (n = 8), and Cd+ L.plantarum (n = 8). Cd (2.04 mg / mL) was orally applicated to Cd and Cd+ L.plantarum groups for 28 days. In addition, active-live L.plantarum (approximately 108 - 109 cfu / mL) was applicated orally to L.plantarum and Cd+ L.plantarum groups at the same time. C group animals received only fresh water and standard rat food during the study. After the applications, animals were decapitated and blood samples were collected for evaluating of some blood parameters (WBC and its different sub-types, RBC, HCT, MCV, MCH, MCHC, and PLT) and also ANAE-positive lymphocyte ratios. WBC counts were determined the highest in the Cd group compared to other experimental groups (p < 0.05). On the other hand, LP administration caused a significant amelioration in WBC counts of Cd+ L.plantarum group animals when compared to Cd group (p < 0.05). Although NEU counts were detected the highest in the Cd group, these counts decreased due to oral L.plantarum administration in Cd+ L.plantarum group (p < 0.05). Increased PLT values due to CD administration were fixed by using LP diet in the Cd+ L.plantarum group compared to the Cd group in the study (p < 0.05). In conclusion, L.plantarum partially affects female rats’ blood parameters from the harmful effects of Cd.