Spray-dried Probiotic adjunct with in vitro acid and bile salt tolerance

Abstract

Probiotic Lactobacillus acidophilus LA1 was encapsulated using spray drying with concentrated skim milk with total solids (17%), and maltodextrin (25%), and gum acacia(15%) as carriers. The encapsulated probiotics were assessed for viability following drying at an inlet temperature of 170°C ±3°C and an outlet temperature of 80°C ± 3°C. The viable countenumerated after drying was 108 log cfu/g. The prepared probiotic adjunct was further assessed for viability through in vitro acidand bile salt exposure assay. The results indicated that the cellcount decreased at lower pH (1, 2) and high incubation periods(1 hr and 2 hr). A similar trend was observed with bile. However, they showed better survivability for different incubation periods at low pH and in high bile salt concentrations. The spray-dried probiotic adjunct was kept for storage at three different temperatures i.e., 8 °C, 25 °C, and 37 °C for eight weeks, and assessed weekly for cell viability. An inverse relationship between cell survival and storage temperature was observed during storage.