High Abundances and Expression Levels of Atypical, Non‐Denitrifier N2O Reductases Drive Strong Microbial N2O Consumption Rates in a Minimally Impacted Mangrove Stand

Author:

Jameson Brett D.12ORCID,Lopez Mark Louie D.3ORCID,Bonderud Matthew3,Stevens Catherine J.24,Juniper S. Kim245,Helbing Caren C.3,Grundle Damian S.16

Affiliation:

1. Bermuda Institute of Ocean Sciences, a Unit of the Julie Ann Wrigley Global Futures Laboratory at Arizona State University St. George's Bermuda

2. School of Earth and Ocean Sciences University of Victoria Victoria BC Canada

3. Department of Biochemistry and Microbiology University of Victoria Victoria BC Canada

4. Department of Biology University of Victoria Victoria BC Canada

5. Ocean Networks Canada Victoria BC Canada

6. School of Ocean Futures School of Earth and Space Exploration Arizona State University Tempe AZ USA

Abstract

AbstractKnowledge of the ecological mechanisms governing N2O cycling in marine sediments lags that of water columns and terrestrial soils, leaving much to be learned about how microbial community dynamics relate to variability in sediment N2O fluxes. The present study assesses these relationships across two distinct environments by focusing on the community structure and activity of N2O reducing microorganisms. The N2O sink capacity of minimally impacted Bermudian mangrove sediments was first estimated using trace‐level microsensors and profile interpretation modeling. Molecular data obtained from these sediments were then compared with those from the Northeast Subarctic Pacific (NESAP) outer continental margin, where previous measurements suggest considerable N2O effluxes. Net N2O uptake was observed for mangrove sediments under ambient and elevated dissolved inorganic nitrogen concentrations (−0.22 ± 0.15 to −0.30 ± 0.26 μmol N2O m−2 d−1), suggesting the microbial potential for N2O consumption exceeded the potential for production via combined nitrification and denitrification. Targeting of bacterial nosZI and nosII gene clusters for quantification using qPCR indicated higher abundance and expression of non‐denitrifier nosZII genes in mangrove sediments demonstrating net N2O uptake. Net N2O production in NESAP sediments was associated with higher abundance and expression of nosZI genes associated with canonical denitrifiers. These results suggest that organisms possessing atypical nosZII genes may act as important N2O scavengers in low‐nitrogen coastal sediments.

Funder

Genome Canada

Genome British Columbia

University of Victoria

Bermuda Institute of Ocean Sciences

Publisher

American Geophysical Union (AGU)

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