Effect of adding L.arginine on some parameters of bull sperms after freezing in liquid nitrogen (-196˚C).

Abstract

In order to investigate the effect of adding L.arginine on poor motile bull sperms, this study was conducted in Artificial Insemination Center of Abou-Ghareeb . 17 ejaculates with poor motile sperms estimated (40% to 55%) were collected by artificial vagina from 5 bulls and extended with Tris-yolk-fructose-glycerol extender supplemented with 0.005 M of L.arginine, cooled for 2 hours, equilibrated for 4 hours and frozen in liquid nitrogen at (-196˚C), then thawed after 48 hours in (37˚C for 30 seconds) . Determination for spermatozoal motility, dead and abnormalities percentages and acrosomal abnormalities percentage were evaluated before and after freezing. Results obtained in this study concluded that L.arginine can be used to activate the motility of poor motile bull sperms, furthermore, it can be used as an adjuvant in bull semen extenders to maintain the viability of spermatozoa after cryopreservation in liquid nitrogen at (-196˚C).