A rapid screening assay for L452R and T478K spike mutations in SARS-CoV-2 Delta variant using high-resolution melting analysis

Author:

Aoki Akira1,Adachi Hirokazu2,Mori Yoko1,Ito Miyabi2,Sato Katsuhiko2,Okuda Kenji2,Sakakibara Toru2,Okamoto Yoshinori1,Jinno Hideto1

Affiliation:

1. Faculty of Pharmacy, Meijo University

2. Aichi Prefectural Institute of Public Health

Publisher

Japanese Society of Toxicology

Subject

Toxicology

Reference14 articles.

1. Aoki, A., Mori, Y., Okamoto, Y. and Jinno, H. (2021): Development of a genotyping platform for SARS-CoV-2 variants using high-resolution melting analysis. J. Infect. Chemother., 27, 1336-1341.

2. Cherian, S., Potdar, V., Jadhav, S., Yadav, P., Gupta, N., Das, M., Rakshit, P., Singh, S., Abraham, P. and Panda, S. (2021): Convergent evolution of SARS-CoV-2 spike mutations, L452R, E484Q and P681R, in the second wave of COVID-19 in Maharashtra, India. bioRxiv, doi: https://doi.org/10.1101/2021.04.22.440932.

3. Davis, C., Logan, N., Tyson, G., Orton, R., Harvey, W., Haughney, J., Perkins, J., Peacock, T.P., Barclay, W.S., Cherepanov, P., Palmarini, M., Murcia, P.R., Patel, A.H., Robertson, D.L., Thomson, E.C. and Willett, B.J. (2021): Reduced neutralisation of the Delta (B.1.617.2) SARS-CoV-2 variant of concern following vaccination. medRxiv, doi: https://doi.org/10.1101/2021.06.23.21259327.

4. Deng, X., Garcia-Knight, M.A., Khalid, M.M., Servellita, V., Wang, C., Morris, M.K., Sotomayor-González, A., Glasner, D.R., Reyes, K.R., Gliwa, A.S., Reddy, N.P., Sanchez San Martin, C., Federman, S., Cheng, J., Balcerek, J., Taylor, J., Streithorst, J.A., Miller, S., Sreekumar, B., Chen, P.Y., Schulze-Gahmen, U., Taha, T.Y., Hayashi, J.M., Simoneau, C.R., Kumar, G.R., McMahon, S., Lidsky, P.V., Xiao, Y., Hemarajata, P., Green, N.M., Espinosa, A., Kath, C., Haw, M., Bell, J., Hacker, J.K., Hanson, C., Wadford, D.A., Anaya, C., Ferguson, D., Frankino, P.A., Shivram, H., Lareau, L.F., Wyman, S.K., Ott, M., Andino, R. and Chiu, C.Y. (2021): Transmission, infectivity, and neutralization of a spike L452R SARS-CoV-2 variant. Cell, 184, 3426-3437.e8.

5. Gorbalenya, A.E., Baker, S.C., Baric, R.S., de Groot, R.J., Drosten, C., Gulyaeva, A.A., Haagmans, B.L., Lauber, C., Leontovich, A.M., Neuman, B.W., Penzar, D., Perlman, S., Poon, L.L., Samborskiy, D.V., Sidorov, I.A., Sola, I. and Ziebuhr, J.; Coronaviridae Study Group of the International Committee on Taxonomy of Viruses. (2020): The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2. Nat. Microbiol., 5, 536-544.

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