Alteration in the Antioxidant Enzymes Activities as Potential Biomarkers for Identification of Stress Caused by Afidopyropen Intoxication in Cyprinus Carpio.

Abstract

This study comparatively investigated the afidopyropen-induced oxidative stress in the fish Cyprinus carpio which is mainly focused on some biochemical parameters such as SOD, CAT, GPx, LPO, ALT, ALP, and GGT activities. Fishes were exposed to different durations (1, 10, 20, and 30 days) in a semi-static method at a sub-lethal concentration of LC50 value. Results show that the duration elicited higher levels of LPO, ALT, and ALP. The LPO levels in the blood were higher in 30 days of exposed fish and this led to significant changes in GPx. The fish exposed for 1 day had found the least increment of 0.71%, and 2.08% in ALT, and ALP activity respectively. The assessment of CAT, GPx, and GGT they were decreased by sub-lethal concentration i.e., 0.2 mg/L of afidopyropen for 10, 20, and 30 days. The results conclude that the afidopyropen has a negative effect on the fish Cyprinus carpio and these differences are evidenced by sensitivity to afidopyropen in the aquatic environment.