Morphogenetic Responses in Anthurium Andreanum (Hort) Cultivars

Abstract

In this study, in vitro morphogenetic responses have been studied in popular Anthurium andreanum cultivars Tropical Red, Acropolis, Sun Glow, Esmeralda, Chaco, Pistachio, Neveda and Safari employing explants of various types and growth stages, through indirect organogenesis. Creamy and compact callus initiated in cut ends along the veins of Leaf lamina with mid rib (LLMR) and petiole with leaf (PL) explants of various cultivars in 30-120 days of inoculation, it was sub cultured and incubated in dark for proliferation. Leaf tip, candle and petioles did not show any kind of response, turned brown and died later. Explants from young pale green leaves with smooth structure showed early response in all cultivars. Callus proliferation rate was 3.0-5.0 times 30-35 days of incubation in various cultivars. Shoot bud initiation and elongation observed after 45-60 days incubation in dark. Number of shoot buds per explants ranged from 30-60 per explants and 8-10 buds attained 1.5-2.5 cm in height. Then, shoots were separated, inoculated in shoot elongation medium and incubated in light. Remaining callus was sub cultured in callus multiplication medium and incubated in dark. Callus and shoot multiplication ratio was 1-2 and 2.5-4.2 times respectively. Shoots attained 4-5.3 cm in height in 45-55 days, rooted and ready for hardening in 65-80 days. Rooted plantlets were hardened in 1:1 ratio soil rite and coco-peat mix in portrays, transferred to beds prepared with coconut coir-pith, 95% plants survived, appeared normal and flowered. In this paper, materials, methodology, results will be discussed.