The Effect of Vitamin D Administration on Leptin, Adiponectin and mRNA MCP-1 Levels in Adipose Tissue of Obese Female Wistar Rats

Author:

Sundari Luh Putu Ratna1ORCID,Bakta Made2ORCID,Astawa Nyoman Mantik3ORCID,Adiatmika Putu Gede1ORCID,Arijana Gusti Kamasan Nyoman4ORCID,Tunas Ketut5

Affiliation:

1. Physiology Department, Faculty of Medicine, Udayana University, Bali, Indonesia

2. Internal Medicine Department, Faculty of Medicine-Sanglah Hospital, Udayana University, Bali, Indonesia

3. Immunology Laboratory, Faculty of Veterinary, Udayana University, Bali Indonesia

4. Histology Department, Faculty of Medicine, Udayana University, Bali, Indonesia

5. Department of Public Health, Faculty of Health, Science and Technology, Dhyana Pura University, Bali, Indonesia

Abstract

In obesity, there is an accumulation of adipocytes which produces adipokine that are pro-inflammatory substance, such as leptin and MCP-1 and anti-inflammatory substance, such as adiponectin, while the bioavailability of vitamin D is decreased. This research aimed to study the effect of vitamin D administration on leptin, MCP-1, and adiponectin levels in adipose tissue rats with obesity. Vitamin D was administered to the obese model of 6-9 months old female Wistar rats. This experiment was a randomized control group design with a post-test group design only. Twenty-seven (27) female obese Wistar rats were included in this study. The animals were divided randomly into 3 groups: 9 rats were given 2400 IU vitamin D (group A), 9 rats were given 800 IU vitamin D (group B) and 9 rats were given a placebo as control (group C). The administration of Vitamin D was given once daily for 8 weeks. The visceral adipose tissue was taken to measure the level of leptin, adiponectin and mRNA MCP-1. Data among groups was analyzed by using one-way ANOVA and followed by LSD test, at a significance level of p <0.05. The lowest level of leptin (1059.15+135.20 pg/ml) and mRNA MCP-1 (2.36 + 0.75 fg/ml) and the highest adiponectin level (3.43 + 0.47 ng/ml) were found in group A. In conclusion, oral administration of vitamin D (2400 IU) decreased pro-inflammatory substances, such as leptin and mRNA MCP-1 and increased anti-inflammatory substances, such as adiponectin, in visceral adipose tissue of obese female Wistar rats.

Publisher

Enviro Research Publishers

Subject

Medicine (miscellaneous),Food Science

Reference35 articles.

1. World Health Organization. Obesity: Preventing and Managing The Global Epidemic..Geneva. 2000;

2. Health Research and Development Unit. National Basic Health Survey 2013. Ministry of Health Republic of Indonesia, 2014.

3. Harbuwono, D. S., Pramono, L. A., Yunir, E., Subekti, I. Obesity And Central Obesity In Indonesia: Evidence From A National Health Survey. Medical Journal of Indonesia. 2018; 27(2): 114-20.

4. Berg, A.H., Scherer, P.E. Adiposa Tissue, Inflammation, And Cardiovascular Disease. Res. 2005; 96 (9): 939–949.

5. Steinberger, J., Steffen, L., Jacobs, D.R, Moran, A., Hong, C.P. Relation Of Leptin To Insulin Resistance Syndrome In Children. Obes Res.2003.;11:1124-1130.

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