Identification of Single Nucleotide Polymorphisms in Selenium-Glutathione Peroxidase (GPX1) Gene-Reference-Cited by-同舟云学术

Identification of Single Nucleotide Polymorphisms in Selenium-Glutathione Peroxidase (GPX1) Gene

Published:2019-11-21 Issue:3 Volume:7 Page:819-827
ISSN:2347-467X
Container-title:Current Research in Nutrition and Food Science Journal
language:
Short-container-title:Curr Res Nutr Food Sci

Author:

Qatatsheh Ala A.¹,Amr Rula²,Al-Holy Murad A.³,Olaimat Amin N.⁴,Ibrahim R.⁵

Affiliation:

1. Department of Clinical Nutrition, College of Applied Health Sciences, Qassim University, P. O. BOX: 6666-Buraidah: 51452, Saudi Arabia and Department of Nursing, Princess Aisha Bint Al-Hussein Faculty of Nursing, Al-Hussein bin Talal University, Ma’an, Jordan

2. Department of Human Nutrition and Dietetics, American University of Madaba , Jordan, Madaba

3. Department of Clinical Nutrition and Dietetics, Faculty of Allied Health Science, The Hashemite University, Zarqa, Jordan

4. Department of Nutrition and Food Science Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt, Jordan

5. Darbour

Abstract

Human genetic variation is quite common with single nucleotide polymorphisms (SNPs) accounting for the majority of the variants. In the present study, primers for amplification of the appropriate part of the human GPX1 gene by polymerase chain reaction (PCR) were designed. The aim of this study was to develop a restriction fragment length polymorphism (RFLP) assay to detect and characterize GPX1 polymorphism in the coding region and validate the assays by sequencing. This study demonstrated that the RFLP method, which can be rapid, is reliable and valid as a tool for identifying the different polymorphisms with a high degree of specificity and sensitivity.

Publisher

Enviro Research Publishers

Subject

Medicine (miscellaneous),Food Science

Reference21 articles.

1. Rotruck J.T., Pope A. L., Ganther H.E., Swanson A.B., Hafeman D.G., Hoekstra W.G. Selenium: Biochemical Role as a Component of Glutathione Geroxidase. Science. 1973; 179: 588-590.

2. Brigelius-Flohe R. Tissue-Specific Functions of Individual Glutathione Peroxidases. Free Radicals in Biology and Medicine. 1999; 27: 951-965.

3. Evenson J.K., Wheeler A.D., Blake S.M., Sunde R.A. Selenoprotein mRNA is expressed in blood at levels comparable to major tissues in rats. Journal of Nutrition. 2004; 134: 2640-2645.

4. Sunde R. The Biochemistry of Selenoproteins. J Am Oil Chem Soc. 1984; 61: 1891-1900.

5. Grossman A.A. Wendel. Non-Reactivity of the Selenoenzyme Glutathione Peroxidase with Enzymatically Hydroperoxidized Phospholipids. European Journal of Biochemistry.1983; 135: 549-552.