Glass Bead-based Genetic Transformation:An Efficient Method for Transformation of Thraustochytrid Microorganisms

Author:

Adachi Takumi12,Sahara Takehiko2,Okuyama Hidetoshi3,Morita Naoki12

Affiliation:

1. Laboratory of Molecular Environmental Microbiology, Graduate School of Agriculture, Hokkaido University

2. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

3. Laboratory of Environmental Molecular Biology, Section of Environmental Biology, Faculty of Environmental Earth Science, Hokkaido University

Publisher

Japan Oil Chemists' Society

Subject

General Chemical Engineering,General Medicine,General Chemistry

Reference12 articles.

1. 1) Lewis, T.E.; Nichols, P.D.; McMeekin, T.A. The biotechnologyical potential of thraustochytrids. Mar. Biotechnol. 1, 580-587 (1999).

2. 2) Kaya, K.; Nakazawa, A.; Matsuura, H.; Honda, D.; Inouye, I.; Watanabe, M. Thraustochytrid Aurantiochytrium sp. 18W-13a accumulates high amounts of squalene. Biosci. Biotechnol. Biochem. 75, 2246-2248 (2011).

3. 3) Aki, T.; Hachida, K.; Yoshinaga, M.; Katai, Y.; Yamasaki, T.; Kawamoto, S.; Kakizono, T.; Maoka, T.; Shigeta, S.; Suzuki, O.; Ono, K. Thraustochytrid as a potential source of caroteneoids. J. Am. Oil Chem. Soc. 80, 789-794 (2003).

4. 4) Sakaguchi, K.; Matsuda, T.; Kobayashi, T.; Ohara, J.; Hamaguchi, R.; Abe, E.; Nagano, N.; Hayashi, M.; Ueda, M.; Honda, D.; Okita, Y.; Taoka, Y.; Sugimoto, S.; Okino, N.; Ito, M. Versatile transformation system that is applicable to both multiple transgene expression and gene targeting for thraustochytrids. Appl. Environ. Microbiol. 78, 3193-3202 (2012).

5. 5) Cheng, R.; Ma, R.; Li, K.; Rong, H.; Lin, X.; Wang, Z.; Yang, S.; Ma, Y. Agrobacterium tumefaciens mediated transformation of marine microalgae Schizochytrium. Microbiol. Res. 167, 179-186 (2012).

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