Detection of Acyl-CoA Derivatized with Butylamide for in vitro Fatty Acid Desaturase Assay
Author:
Affiliation:
1. Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
Publisher
Japan Oil Chemists' Society
Subject
General Chemical Engineering,General Medicine,General Chemistry
Link
https://www.jstage.jst.go.jp/article/jos/65/2/65_ess15257/_pdf
Reference25 articles.
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2. 2) Wallis, J. G.; Watts, J. L.; Browse, J. Polyunsaturated fatty acid synthesis: What will they think of next? Trends Biochem. Sci. 27, 467-473 (2002).
3. 3) Sakuradani, E.; Kobayashi, M.; Shimizu, S. Δ6-Fatty acid desaturase from an arachidonic acid-producing Mortierella fungus: Gene cloning and its heterologous expression in a fungus, Aspergillus. Gene 238, 445-453 (1999).
4. 4) Sayanova, O.; Smith, M. A.; Lapinskas, P.; Stobart, A. K.; Dobson, G.; Christie, W. W.; Shewry, P. R.; Napier, J. A. Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of Δ6-desaturated fatty acids in transgenic tobacco. Proc. Natl. Acad. Sci. U. S. A. 94, 4211-4216 (1997).
5. 5) Cho, H. P.; Nakamura, M.; Clarke, S. D. Cloning, expression, and fatty acid regulation of the human Δ5 desaturase. J. Biol. Chem. 274, 37335-37339 (1999).
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