Experimental substantiation of the effectiveness of antimicrobial varnish for the treatment of finished prostheses in the prevention of prosthetic stomatitis

Abstract

Annotation. Denture stomatitis is considered to be a classic form of biofilm-associated infection, therefore, the measures leading to inhibition of biofilm formation on denture surface take a significant place among the different strategies to prevent this infection. Candida spp. are known as leading causes of denture stomatitis, so the aim of our research was to determine the influence of the original hydrophobic antimicrobial coating on the adhesive and biofilm-forming ability of oral Candida fungi, which were isolated from patients with manifestation of denture stomatitis. Spectrophotometry and quantitative microbiological assay were used to study adhesion and biofilm formation by clinical isolates of fungi on intact surfaces and those, which were covered with antimicrobial coating; results were processed by detection of statistical data with Microsoft Excell program, validity of the difference between the control and experimental samples was proved according to Student criteria (р≤0,05). It was established with spectrophotometry assay that among 25 clinical isolates of Candida spp., the vast majority (18 strains) were weak producers of biofilm (the coefficient of biofilm formation intensity (ROD) was 1.13-1.5), 5 strains were more potential biofilm producers (average ROD was more than 2.3). According to the obtained data, clinical isolates of Candida albicans formed a biofilm more intensively than other species of isolated candida. It was demonstrated that the biofilm-forming fungi (average ROD was 2.36) were unable to produce a biofilm on surfaces covered with antimicrobial coating, which was proved by a decrease of coefficient of biofilm formation intensity to 0.7. By quantitative microbiological assay it was determined that oral isolates of Candida spp. were able to form a biofilm on the denture resin samples, however, adhesion and biofilm production reduced by 1.7 and 2.8 times, respectively, on the surface, which was treated with the antimicrobial coating. In the future, it is planned to conduct clinical trial to detect effectiveness of dentures, which are covered with antimicrobial coating, in denture stomatitis prevention.