Changes in colonization resistance and composition of the microflora of the colon cavity of white rats with oral administration of calcium acetylacetonate

Abstract

Annotation. The purpose of the study is to experimentally reveal the effect of calcium acetylacetonate at a dose of 250 mg/kg on colonization resistance and on changes in the composition of the microflora of the colon cavity of white rats 8 hours after its single administration directly into the stomach. The experiment was conducted on 34 outbred white rats weighing 200-220 g, divided into two groups. The control group was not administered calcium acetylacetonate. The microbiological research method is applied. It was found that alcium acetylacetonate at a dose of 250 mg/kg leads not only to changes in the qualitative composition of the microbiota of the contents of the colon cavity, but also to significant changes in the quantitative composition and deep changes in the relationships between the representatives of the main microbiota, as well as taxa of additional and residual microflora. Thus, the population level of the most physiologically useful bacteria of the genus Bifidobacterium, bacteria of the genus Lactobacillus (by 57.4%), Bacteroides (by 21.5%), Escherichia (by 40.5%). The above dose of calcium acetylacetonate contributes to the elimination from the colon cavity of representatives of the main microbiota – bacteria of the genera Eubacterium, Peptostreptococcus, Enterococcus, as well as transient streptobacteria, which exhibit significant antagonistic activity against pathogenic and opportunistic enterobacteria, staphylococci, and others. Based on the obtained data, there is a need for research aimed at the search and selection of agents that have a decontaminating and regulatory effect on the qualitative composition of the microbiota of the colon cavity after oral ingestion of calcium acetylacetonate.