AN ACCELERATED METHOD OF DIPHTHERIA GENE DIAGNOSTICS BASED ON ISOTHERMAL AMPLIFICATION TO DETECT DNA OF THE CAUSATIVE AGENT

Author:

Borisova O. Yu.1,Pimenova A. S.2,Chaplin A. V.1,Kafarskaya L. I.2,Afanasiev S. S.2,Aleshkin V. A.2,Aleshkin A. V.2,Afanasiev M. S.3,Karaulov A. V.3

Affiliation:

1. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Pirogov Russian National Research Medical University

2. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology

3. Sechenov First Moscow State Medical University

Publisher

Central Research Institute for Epidemiology

Subject

General Medicine

Reference12 articles.

1. Aravena-Roman M., Bowman R., O'Neill G. Polymerase chain reaction for the detection of toxigenic Corynebacterium diphtheriae. Pathology. 1995, 27 (1): 71-73. PMID: 7603758.

2. Efstratiou A., Engler K. H., Dawes C. S. et al. Comparison of phenotypic and genotypic methods for detection of diphtheria toxin among isolates of pathogenic Corynebacteria. J. Clin. Microbiol. 1998, 36: 3173-3177.

3. Mancini F., Monaco M., Pataracchia M. et al. Identification and molecular discrimination of toxigenic and nontoxigenic diphtheria Corynebacterium strains by combined real-time polymerase chain reaction assays. Diagn. Microbiol. Infect. Dis. 2012, 73 (2): 111-120.

4. Mikhailovich V.M., Melnikov V.G., Mazurova I.K. et al. Application of PCR for detection of toxigenic Corynebacterium diphtheriae strains isolated during the Russian diphtheria epidemic, 1990 through 1994. J. Clin. Microbiol. 1995, 33 (11): 3061-3063.

5. Mothershed E.A., Cassiday P.K., Pierson K. et al. Development of a real-time fluorescence PCR assay for rapid detection of the diphtheria toxin gene. J. Clin. Microbiol. 2002, 40 (12): 4713-4719.

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