Silent NMDA Receptor–Mediated Synapses Are Developmentally Regulated in the Dorsal Horn of the Rat Spinal Cord

Abstract

In vitro whole cell patch-clamp recording techniques were utilized to study silent pure– N-methyl-d-aspartate (NMDA) receptor–mediated synaptic responses in lamina II (substantia gelatinosa, SG) and lamina III of the spinal dorsal horn. To clarify whether these synapses are present in the adult and contribute to neuropathic pain, transverse lumbar spinal cord slices were prepared from neonatal, naive adult and adult sciatic nerve transected rats. In neonatal rats, pure-NMDA receptor–mediated excitatory postsynaptic currents (EPSCs) were elicited in SG neurons either by focal intraspinal stimulation ( n = 15 of 20 neurons) or focal stimulation of the dorsal root ( n = 2 of 7 neurons). In contrast, in slices from naive adult rats, no silent pure-NMDA EPSCs were recorded in SG neurons following focal intraspinal stimulation ( n = 27), and only one pure-NMDA EPSC was observed in lamina III ( n = 23). Furthermore, in rats with chronic sciatic nerve transection, pure-NMDA EPSCs were elicited by focal intraspinal stimulation in only 2 of 45 SG neurons. Although a large increase in Aβ fiber evoked mixed α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and NMDA receptor–mediated synapses was detected after sciatic nerve injury, Aβ fiber–mediated pure-NMDA EPSCs were not evoked in SG neurons by dorsal root stimulation. Pure-NMDA receptor–mediated EPSCs are therefore a transient, developmentally regulated phenomenon, and, although they may have a role in synaptic refinement in the immature dorsal horn, they are unlikely to be involved in receptive field plasticity in the adult.