Denopamine, a β1-adrenergic agonist, increases alveolar fluid clearance in ex vivo rat and guinea pig lungs

Abstract

The effect of denopamine, a selective β1-adrenergic agonist, on alveolar fluid clearance was determined in both ex vivo rat and guinea pig lungs. Alveolar fluid clearance was measured by the progressive increase in the concentration of Evans blue-labeled albumin over 1 h at 37°C. Denopamine (10−6 to 10−3 M) increased alveolar fluid clearance in a dose-dependent manner in ex vivo rat lungs. Denopamine also stimulated alveolar fluid clearance in guinea pig lungs. Atenolol, a selective β1-adrenergic antagonist, and amiloride, a sodium channel inhibitor, inhibited denopamine-stimulated alveolar fluid clearance. The potency of denopamine was similar to that of similar doses of isoproterenol or terbutaline. Short-term hypoxia (100% nitrogen for 1–2 h) did not alter the stimulatory effect of denopamine. Denopamine (10−4, 10−3 M) increased intracellular adenosine 3′,5′-cyclic monophosphate levels in cultured rat alveolar type II cells. In summary, denopamine, a selective β1-adrenergic agonist, stimulates alveolar fluid clearance in both ex vivo rat and guinea pig lungs.