Validity of NIR spectroscopy for quantitatively measuring muscle oxidative metabolic rate in exercise

Abstract

The purpose of this study was to examine the validity of the quantitative measurement of muscle oxidative metabolism in exercise by near-infrared continuous-wave spectroscopy (NIRcws). Twelve male subjects performed two bouts of dynamic handgrip exercise, once for the NIRcws measurement and once for the 31P-magnetic resonance spectroscopy (MRS) measurement as a standard measure. The resting muscle metabolic rate (RMRmus) was independently measured by 31P-MRS during 15 min of arterial occlusion at rest. During the first exercise bout, the quantitative value of muscle oxidative metabolic rate at 30 s postexercise was evaluated from the ratio of the rate of oxyhemoglobin/myoglobin decline measured by NIRcws during arterial occlusion 30 s after exercise and the rate at rest. Therefore, the absolute values of muscle oxidative metabolic rate at 30 s after exercise [V˙o 2NIR(30)] was calculated from this ratio multiplied by RMRmus. During the second exercise bout, creatine phosphate (PCr) resynthesis rate was measured by 31P-MRS at 30 s postexercise [Q(30)] under the same conditions but without arterial occlusion postexercise. To determine the validity of NIRcws, V˙o 2NIR(30) was compared with Q(30). There was a significant correlation betweenV˙o 2NIR(30), which ranged between 0.018 and 0.187 mM ATP/s, and Q(30), which ranged between 0.041 and 0.209 mM ATP/s ( r = 0.965, P < 0.001). This result supports the application of NIRcws to quantitatively evaluate muscle oxidative metabolic rate in exercise.