Enteropathogenic E. coli attenuates secretagogue-induced net intestinal ion transport but not Cl− secretion

Author:

Hecht Gail1,Koutsouris Athanasia1

Affiliation:

1. Department of Medicine, Section of Digestive and Liver Diseases, University of Illinois and West Side Veterans Affairs Medical Center, Chicago, Illinois 60612

Abstract

Enteric bacterial pathogens often increase intestinal Cl secretion. Enteropathogenic Escherichia coli(EPEC) does not stimulate active ion secretion. In fact, EPEC infection decreases net ion transport in response to classic secretagogues. This has been presumed to reflect diminished Cl secretion. The aim of this study was to investigate the influence of EPEC infection on specific intestinal epithelial ion transport processes. T84 cell monolayers infected with EPEC were used for these studies. EPEC infection significantly decreased short-circuit current ( I sc) in response to carbachol and forskolin, yet125I efflux studies revealed no difference in Cl channel activity. There was also no alteration in basolateral K+ channel or Na+-K+-2Clcotransport activity. Furthermore, net36Clflux was not decreased by EPEC. No alterations in either K+ or Na+ transport could be demonstrated. Instead, removal of basolateral bicarbonate from uninfected monolayers yielded an I sc response approximating that observed with EPEC infection, whereas bicarbonate removal from EPEC-infected monolayers further diminished I sc. These studies suggest that the reduction in stimulated I sc is not secondary to diminished Clsecretion. Alternatively, bicarbonate-dependent transport processes appear to be perturbed.

Publisher

American Physiological Society

Subject

Physiology (medical),Gastroenterology,Hepatology,Physiology

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