Electrical characteristics of inward-rectifying K+ channels in isolated bullfrog oxyntic cells

Abstract

The properties of K+ channels in the isolated oxyntic cells of the bullfrog (Rana catesbeiana) were investigated using the patch-clamp method. Two types of K+ channels on the basolateral membrane were identified on the basis of their electrophysiological and pharmacological properties. The K+ channel most frequently observed has a single-channel conductance of 61.0 +/- 2.9 pS (n = 10) and is activated by an increase in intracellular Ca2+. The other K+ channel has a single-channel conductance of 30.3 +/- 2.7 pS (n = 7), which is activated by adenosine 3',5'-cyclic monophosphate (cAMP). The physiological and pharmacological characteristics common to the two K+ channels are inward-going rectification with a high selectivity for K+ and indirect inhibition by omeprazole. The inward rectification is controlled by intracellular Mg2+ in such a way that the more Mg2+ is applied intracellularly, the more their inward-rectifying property is enhanced. The finding that bethanechol and cAMP increase the open probability of these K+ channels as well as activating the acid secretion indicates that there may be a relationship between these two processes in the oxyntic cells.