Contribution of surface epithelial cells to total conductance of Necturus gastric fundus mucosa

Author:

Kottra G.1,Iacovelli C.1,Caroppo R.1,Curci S.1,Bakos P.1,Fromter E.1

Affiliation:

1. Zentrum der Physiologie, Klinikum der J. W. Goethe-Universitat,Frankfurt, Germany.

Abstract

Microelectrode techniques were used to quantify the contribution of surface epithelial cells (SEC) to transepithelial conductance (gt) of Necturus gastric fundus mucosa. Transepithelial voltage (Vt) and resistance (Rt) as well as the basolateral cell membrane potential (Vb) and voltage divider ratio of SEC were measured. Freshly mounted preparations did not respond to luminal amiloride (10 microM), but within 2-3 h a significant response developed (delta Vt = 3.8 +/- 1.2 mV, delta Rt = 63 +/- 23 omega cm2, and delta Vb = -6.9 +/- 1.3 mV), indicating activation of an apical Na+ conductance in SEC. Using circuit analysis equations, we calculate that SEC contribute 10.4% to gt under control conditions and 13.0% after Na+ conductance activation. Histamine (0.1 mM), which stimulates the oxyntopeptic cells (OC), increased Vt and decreased Rt but did not significantly alter the membrane resistances of SEC. As a result, the contribution of SEC to gt fell to 7.4 or 9.3%, respectively. The data confirm that SEC are poorly permeable and that the major conductance path across gastric mucosa leads through OC in the glands. The reason for the protracted in vitro activation of the apical Na+ conductance in SEC is not known.

Publisher

American Physiological Society

Subject

Physiology (medical),Gastroenterology,Hepatology,Physiology

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Role of Na-K-2Cl cotransporter-1 in gastric secretion of nonacidic fluid and pepsinogen;American Journal of Physiology-Gastrointestinal and Liver Physiology;2005-09

2. Helicobacter pyloricytotoxin VacA increases alkaline secretion in gastric epithelial cells;American Journal of Physiology-Gastrointestinal and Liver Physiology;2001-12-01

3. Is resting state HCO3- secretion in frog gastric fundus mucosa mediated by apical Cl(-)-HCO3- exchange?;The Journal of Physiology;1997-03-15

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