Correlation of recruitment order with axonal conduction velocity for supraspinally driven diaphragmatic motor units

Abstract

Spontaneous activities of pairs of single diaphragmatic motor units (MUs) were recorded via two electrodes in anesthetized cats, ventilated with CO2 added to the inspired gas, which slightly enhanced respiratory drive (endtidal CO2 less than 6%). These MUs were characterized by their axonal conduction velocities (CVs) and relative onset times (defined as the time after onset of phrenic nerve activity until the MU began discharging divided by the duration of inspiration). Motor unit axonal CV was estimated by the conduction time and the distance between two points on the phrenic nerve. Results were compared from two experimental preparations: one with dorsal roots intact and the other with dorsal roots transected bilaterally between fourth (C4) and seventh (C7) cervical segments. Estimated mean CV for phrenic MUs was 46.2 m/s(n = 180 MU). Motor units were classified as early and late recruited MUs depending on their relative onset times. We correlated MU axonal CV with its relative recruitment time. A highly significant (P less than 0.0001), positive correlation between axonal CV and relative recruitment time was established for those diaphragmatic MUs recruited with this respiratory drive. Correlation coefficients were r = 0.70 for intact animals, r = 0.72 for dorsal rhizotomized animals, and r = 0.72 overall population. For pairs of MUs, the CV of the earlier recruited unit was compared with the CV of the later recruited unit. In 96% of pairs from intact animals and 92% of pairs from dorsal rhizotomized animals, the first MU had a lower CV than the MU recruited later. Difference in relative times of recruitment was directly related to difference in axonal CVs. However, a portion of the motor pool with high-axonal CVs was not sampled. Under conditions of these experiments, afferent input in cervical dorsal roots, including that from diaphragmatic receptors, did not influence the distribution of MU relative onset times. Further, a similar proportion of MU pairs wherein the earlier recruited MU had a CV lower than the later recruited unit was observed in intact and dorsal rhizotomized animals. We also cross-correlated 31 pairs of simultaneously recorded MUs to assess common input onto phrenic motoneurons. Common input was characterized by the presence of peaks having widths of greater than or equal to 3 ms in the cross-correlation histograms (CCHs) and occurring within 20 ms of the trigger event. Peaks were judged significant if the bin with the largest number of occurrences was significantly greater than base line and if neighboring bins were above base line.(ABSTRACT TRUNCATED AT 400 WORDS)