Affiliation:
1. The Jackson Laboratory, Bar Harbor, Maine 04609
2. Case Western Reserve University, Cleveland, Ohio 44109
3. Department of Medicine, Harvard Medical School, Gastroenterology Division, Brigham and Women’s Hospital and Harvard Digestive Diseases Center, Boston, Massachusetts 02115
Abstract
Quantitative trait locus (QTL) mapping was used to locate genes that determine the difference in cholesterol gallstone disease between the gallstone-susceptible strain C57L/J and the gallstone-resistant strain AKR/J. Gallstone weight was determined in 231 male (AKR × C57L) F1× AKR backcross mice fed a lithogenic diet containing 1% cholesterol, 0.5% cholic acid, and 15% butterfat for 8 wk. Mice having no stones and mice having the largest stones were genotyped at ∼20-cM intervals to find the loci determining cholesterol gallstone formation. The major locus, Lith1, mapped near D2Mit56 and was confirmed by constructing a congenic strain, AK.L- Lith1s. Another locus, Lith2, mapped near D19Mit58 and was also confirmed by constructing a congenic strain AK.L- Lith2s. Other suggestive, but not statistically significant, loci mapped to chromosomes 6, 7, 8, 10, and X. The identification of these Lith genes will elucidate the pathophysiology of cholesterol gallstone formation.
Publisher
American Physiological Society
Reference42 articles.
1. Different susceptibilities to the formation of cholesterol gallstones in mice
2. Apstein MD and Carey MC.Gallstones. In:Office Practice of Medicine(3rd ed.). Philadelphia: Saunders, 1994, p. 277–295.
3. Factors associated with gallstone disease in the MICOL experience
4. Bouchard G, Chao HC, Lammert F, Wang DQH, Carey MC, and Paigen B.Cmoatis a candidate gene forLith2, a cholesterol gallstone gene found on chromosome 19 in inbred mice (Abstract).Hepatology28: 1360, 1998.
5. High-resolution maps of the murine Chromosome 2 region containing the cholesterol gallstone locus, Lith1
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