Affiliation:
1. Department of Biological, Geological, and Environmental Sciences, Cleveland State University, Cleveland 44115
2. Center for Molecular Genetics, Cleveland Clinic Foundation, Cleveland, Ohio 44195
3. Department of Molecular Cardiology, Cleveland Clinic Foundation, Cleveland, Ohio 44195
4. Department of Cardiovascular Medicine, Cleveland Clinic Foundation, Cleveland, Ohio 44195
Abstract
Genetic factors increase the risk to coronary artery disease (CAD). To date, a limited number of genes that potentially contribute to development of CAD have been identified. In this study, we have performed large-scale gene expression analysis of ∼12,000 human genes in nine severely atherosclerotic and six nonatherosclerotic human coronary arteries using oligonucleotide microarrays. Fifty-six genes showed differential expression in atherosclerotic coronary artery tissues; expression of 55 genes was increased in atherosclerotic coronary arteries, whereas only one gene, GST, encoding a reducing agent, showed downregulated expression. The expression data of selected genes were validated by quantitative RT-PCR analysis as well as immunostaining. The associations of 49 genes with CAD appear to be novel, and they include genes encoding ICAM-2, PIM-2, ECGF1, fusin, B cell activator ( BL34, GOS8), Rho GTPase activating protein-4, retinoic acid receptor responder, β2-arrestin, membrane aminopeptidase, cathepsins K and H, MIR-7, TNF-α-induced protein 2 (B94), and flavocytochrome 558. In conclusion, we have identified 56 genes whose expression is associated with CAD, and 49 of them may represent new genes linked to CAD.
Publisher
American Physiological Society
Cited by
69 articles.
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