Altered mRNA abundance of ASB15 and four other genes in skeletal muscle following administration of β-adrenergic receptor agonists

Author:

McDaneld Tara G.1,Hancock Deana L.2,Moody Diane E.1

Affiliation:

1. Department of Animal Sciences, Purdue University, West Lafayette 47907-2054

2. Elanco Animal Health, Greenfield, Indiana 46140

Abstract

β-Adrenergic receptor agonists (BA) stimulate skeletal muscle growth. However, downstream signaling pathways that facilitate this effect remain poorly defined. Objectives of this study were to identify genes differentially expressed after administration of a novel BA and to evaluate the expression of one of those genes in additional models of skeletal muscle growth. Differentially expressed gene fragments were identified through differential display of skeletal muscle biopsies from five steers 24 h after administration of the BA. Five gene fragments designated DD53, DD143, DD163, DD209, and DD214 were identified. Tissue distribution of these genes was evaluated by RT-PCR. While DD53, DD163, DD209, and DD214 were expressed across tissues, DD143 mRNA expression was most abundant in skeletal muscle. DD143, later identified as bovine ASB15, was evaluated in rats following administration of anabolic compounds. Thirteen 7-wk-old female rats were randomly assigned to each of four treatment groups including: control, clenbuterol, trenbolone acetate (TBA), and growth hormone (GH). Changes in rat Asb-15 mRNA were measured at 30 min, 12 h, and 24 h following intraperitoneal injections of each compound. Clenbuterol treatment decreased Asb-15 mRNA in skeletal muscle at 12 and 24 h ( P < 0.01) and also decreased mRNA in lung at 12 h ( P < 0.05). TBA and GH treatments did not alter Asb-15 mRNA in any of the tissues evaluated ( P > 0.10). These results are the first to associate an Asb gene family member with muscle growth or BA administration and suggest a potential role for ASB15 in β-agonist-induced skeletal muscle hypertrophy.

Publisher

American Physiological Society

Subject

Genetics,Physiology

Reference41 articles.

1. Basic local alignment search tool

2. Anderson DB, Veenhuizen EL, Schroeder AL, Jones DJ, and Hancock DL. The use of phenethanolamines to reduce fat and increase leanness in meat animals. In: Proceedings of Symposium on Fat and Cholesterol Reduced. Advances in Applied Biotechnology Series, edited by Haberstroh C and Morris CE. New Orleans: Portfolio, 1991, p. 43–73.

3. Effect of β-agonists on expression of calpain and calpastatin activity in skeletal muscle

4. Ankyrins and cellular targeting of diverse membrane proteins to physiological sites

5. The Human Adult Skeletal Muscle Transcriptional Profile Reconstructed by a Novel Computational Approach

Cited by 33 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3