Author:
Åkerblad Peter,Månsson Robert,Lagergren Anna,Westerlund Simonetta,Basta Barbro,Lind Ulrika,Thelin Anders,Gisler Ramiro,Liberg David,Nelander Sven,Bamberg Krister,Sigvardsson Mikael
Abstract
Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)γ2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPARγ2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPARγ2 induce adipocyte differentiation with comparable kinetics and efficiency.
Publisher
American Physiological Society
Cited by
49 articles.
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