Serotonergic Modulation of a Voltage-Gated Calcium Current in Parapodial Swim Muscle From Aplysia brasiliana

Abstract

Laurienti, P. J. and J. E. Blankenship. Serotonergic modulation of a voltage-gated calcium current in parapodial swim muscle from Aplysia brasiliana. J. Neurophysiol. 77: 1496–1502, 1997. Here we describe the effects of serotonin (5-HT) on dissociated parapodial muscle fibers from Aplysia brasiliana. 5-HT has previously been implicated as a modulatory transmitter at the parapodial neuromuscular junction. Exogenously applied or endogenously released 5-HT increases the amplitude of motoneuron-induced excitatory junctional potentials and contractions in parapodial muscle. Exogenously applied 5 μM 5-HT increases the amplitude of a voltage-gated inward calcium current in isolated muscle fibers by an average of 42% in response to a voltage step from −70 to −10 mV. The amplitude of the inward current was increased at all voltages tested, with the peak increase occurring between −30 and −20 mV. The dihydropyridine calcium channel antagonist nifedipine (10 μM) blocked this effect of 5-HT. The data indicate that 5-HT increases a previously identified calcium current in parapodial muscle fibers that is similar to the vertebrate L-type current. Although several types of K+ channels exist in these fibers, including Ca2+-dependent K+ channels, the results suggest that 5-HT has little effect on these currents. Parapodial muscle contractions during swimming behavior occur in response to bursts of motoneuron action potentials that produce graded muscle depolarizations that occur over a 1- to 2-s period rather than being instantaneous or rapid responses as might be produced by one or two action potentials or a brief voltage step. With the use of 1-s voltage ramps, we attempted to mimic physiological depolarization and demonstrate that 5-HT is able to increase the amplitude of the inward calcium current. The data presented in this paper provide evidence that 5-HT increases the Ca2+ current, which may be one mechanism by which 5-HT modulates muscle contractions during swim behavior.