Effect of hyposmotic challenge on microvillous membrane potential in isolated human placental villi

Abstract

This study examined the effect of hyposmotic solutions on the syncytiotrophoblast microvillous membrane potential ( E m) in mature intermediate villi isolated from term human placentas. When villi were exposed to a control solution (280 mosmol/kgH2O; 116 mM NaCl) and then to either a 138-hyposmotic (138 mosmol/kgH2O; 37 mM NaCl) or 170-hyposmotic (170 mosmol/kgH2O; 55 mM NaCl) solution, there was a significant hyperpolarization of E m (−5.1 ± 1.5 mV, P < 0.01 and −5.0 ± 0.5 mV, P < 0.001, respectively; n = 10), which was reversible on removal of the hyposmotic stimulus. Low-NaCl (37 and 55 mM) solutions made isosmotic with control (i.e., 280 mosmol/kgH2O) by addition of raffinose did not significantly alter E m, suggesting that reducing NaCl concentration per se had no effect on E m. Exposure to 170-hyposmotic solution in the presence of 5 mM BaCl2 depolarized E m by +4.1 ± 0.7 mV ( P < 0.001, n = 6); BaCl2 similarly depolarized E m when added in control solution (+5.6 ± 1.1 mV, n= 5). Exposure to 170-hyposmotic solution containing 1 mM DIDS hyperpolarized E mby −9.0 ± 1.7 mV ( P < 0.001, n = 5). This degree of hyperpolarization was significantly greater than that observed in hyposmotic solution alone ( P < 0.01) but was not different from the hyperpolarization when DIDS was added to control solution (−7.4 ± 0.2 mV, n = 6). We conclude 1) that Ba2+-sensitive K+ conductances and DIDS-sensitive anion conductances contribute to the resting potential of the syncytiotrophoblast microvillous membrane and 2) that the syncytiotrophoblast microvillous membrane responds to a hyposmotic stimulus by activating both Ba2+-sensitive K+ and DIDS-sensitive anion conductances.