Renal cortical and medullary blood flow responses to altered NO availability in humans

Abstract

The objective of this study was to quantify regional renal blood flow in humans. In nine young volunteers on a controlled diet, the lower abdomen was CT-scanned, and regional renal blood flow was determined by positron emission tomography (PET) scanning using H215O as tracer. Measurements were performed at baseline, during constant intravenous infusion of nitric oxide (NO) donor glyceryl nitrate and after intravenous injection of NO synthase inhibitor Nω-monomethyl-l-arginine (l-NMMA). Using the CT image, the kidney pole areas were delineated as volumes of interest (VOI). In the data analysis, tissue layers with a thickness of one voxel were eliminated stepwise from the external surface of the VOI (voxel peeling), and the blood flow subsequently was determined in each new, reduced VOI. Blood flow in the shrinking VOIs decreased as the number of cycles of voxel peeling increased. After 4–5 cycles, blood flow was not reduced further by additional voxel peeling. This volume-insensitive flow was measured to be 2.30 ± 0.17 ml·g tissue−1·min−1during the control period; it increased during infusion of glyceryl nitrate to 2.97 ± 0.18 ml·g tissue−1·min−1( P < 0.05) and decreased after l-NMMA injection to 1.57 ± 0.17 ml·g tissue−1·min−1( P < 0.05). Cortical blood flow was 4.67 ± 0.31 ml·g tissue−1·min−1during control, unchanged by glyceryl nitrate, and decreased after l-NMMA [3.48 ± 0.23 ml·(g·min)−1, P < 0.05]. PET/CT scanning allows identification of a renal medullary region in which the measured blood flow is 1) low, 2) independent of reduction in the VOI, and 3) reactive to changes in systemic NO supply. The technique seems to provide indices of renal medullary blood flow in humans.