Sympathetic activity in early renal posttransplantation hypertension in rats

Abstract

The contribution of elevated sympathetic activity to the development of renal posttransplantation hypertension was investigated. F1 hybrids (F1H) from spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY) were transplanted with either an SHR or an F1H kidney and bilaterally nephrectomized. Three weeks after transplantation, sympathetic activity was assessed by measuring adrenal tyrosine hydroxylase (TH) mRNA content and recording splanchnic nerve activity (SNA) in conscious animals. To investigate the dependence of arterial pressure on sympathetic activity, animals were treated with the α2-adrenoceptor agonist guanabenz intracerebroventricularly. Mean arterial pressure (MAP) was 143 ± 4 mmHg in recipients of an SHR kidney ( n = 15) versus 110 ± 3 mmHg in recipients of an F1H kidney ( n = 10; P < 0.001). Adrenal TH mRNA content was 1.93 ± 0.15 fmol/μg total RNA in recipients of an SHR kidney versus 1.96 ± 0.17 fmol/μg total RNA in recipients of an F1H kidney (not significant). SNA did not differ significantly between recipients of an SHR kidney ( n = 8) and recipients of an F1H kidney ( n = 7) in terms of frequency and amplitude of synchronized nerve discharges. In response to cumulative intracerebroventricular administration of 10 and 20 μg guanabenz, SNA fell to 51 ± 5% of control in recipients of an SHR kidney versus 44 ± 6% of control in recipients of an F1H kidney (not significant) accompanied by a slight fall in MAP in either group. The results suggest that elevated sympathetic activity is not a major contributor to the development of renal posttransplantation hypertension.