LPS-induced liver injury ind-galactosamine-sensitized mice requires secreted TNF-α and the TNF-p55 receptor

Author:

Nowak Monika1,Gaines Gregory C.1,Rosenberg Jason1,Minter Rebecca1,Bahjat F. R.1,Rectenwald John1,MacKay Sally L. D.1,Edwards Carl K.2,Moldawer Lyle L.1

Affiliation:

1. Department of Surgery, University of Florida College of Medicine, Gainesville, Florida 32610; and

2. Amgen, Thousand Oaks, California 91320

Abstract

Lipopolysaccharide andd-galactosamine induced lethality and apoptotic liver injury is dependent on endogenously produced tumor necrosis factor (TNF)-α. The present study was undertaken to determine whether membrane-associated or secreted TNF-α signaling through the p55 or p75 receptor was responsible for survival and hepatic injury after lipopolysaccharide administration ind-galactosamine-sensitized mice. Transgenic mice expressing null forms of TNF-α, the p55 and p75 receptor, and mice expressing only a cell-associated form of TNF-α were challenged with 8 mgd-galactosamine and 100 ng lipopolysaccharide. Mortality and apoptotic liver injury were only seen in wild-type and p75 knockout mice. p75 Knockout mice had significantly higher concentrations of plasma TNF-α than any other experimental group ( P ≤ 0.05) and tended to have the highest mortality and liver injury. In contrast, p55 and TNF-α knockout mice and animals expressing only a cell-associated form of TNF-α exhibited no mortality or liver injury. We conclude that survival and apoptotic liver injury in response to lipopolysaccharide and d-galactosamine are dependent exclusively on secreted TNF-α signaling through the p55 receptor.

Publisher

American Physiological Society

Subject

Physiology (medical),Physiology

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