Hepatoportal bumetanide-sensitive K+-sensor mechanism controls urinary K+ excretion

Abstract

To determine whether a K+-sensor mechanism exists in the hepatoportal region, periarterial hepatic afferent nerve activity responses to intraportal injection of KCl were examined in anesthetized rats. Hepatic afferent nerve activity increased in response to intraportal injection in a K+ concentration-dependent manner, and the increase was attenuated by inhibition of the Na+-K+-2Cl− cotransporter by bumetanide in a dose-dependent manner. These results suggest that a bumetanide-sensitive K+-sensor mechanism exists in the hepatoportal region. Stimulation of this mechanism by intraportal KCl infusion elicited an immediate and powerful kaliuresis with no significant change in the plasma K+ concentration; this was significantly greater than the kaliuresis induced by intravenous KCl infusion and was attenuated by severing the periarterial hepatic nervous plexus. These results indicate that a hepatoportal bumetanide-sensitive K+-sensor mechanism senses the portal venous K+ concentration and that stimulation of this sensor mechanism causes kaliuresis, which is mainly mediated by the periarterial hepatic nervous plexus.