Differential effects of repetitive activity on sarcoplasmic reticulum responses in rat muscles of different oxidative potential

Abstract

We investigated the hypothesis that muscles of different oxidative potential would display differences in sarcoplasmic reticulum (SR) Ca2+handling responses to repetitive contractile activity and recovery. Repetitive activity was induced in two muscles of high oxidative potential, namely, soleus (SOL) and red gastrocnemius (RG), and in white gastrocnemius (WG), a muscle of low oxidative potential, by stimulation in adult male rats. Measurements of SR properties, performed in crude homogenates, were made on control and stimulated muscles at the start of recovery (R0) and at 25 min of recovery (R25). Maximal Ca2+-ATPase activity ( Vmax, μmol·g protein−1·min−1) at R0 was lower in stimulated SOL (105 ± 9 vs. 135 ± 7) and RG (269 ± 22 vs. 317 ± 26) and higher ( P < 0.05) in WG (795 ± 32 vs. 708 ± 34). At R25, Vmaxremained lower ( P < 0.05) in SOL and RG but recovered in WG. Ca2+uptake, measured at 2,000 nM, was depressed ( P < 0.05) in SOL and RG by 34 and 13%, respectively, in stimulated muscles at R0 and remained depressed ( P < 0.05) at R25. In contrast, Ca2+uptake was elevated ( P < 0.05) in stimulated WG at R0 by 9% and remained elevated ( P < 0.05) at R25. Ca2+release, unaltered in SOL and RG at both R0 and R25, was increased ( P < 0.05) in stimulated WG at both R0 and R25. We conclude that SR Ca2+-handling responses to repetitive contractile activity and recovery are related to the oxidative potential of muscle.