Aldosterone suppresses expression of an avian colonic sodium-glucose cotransporter

Abstract

Transport in the colon of the domestic fowl switches from sodium-linked hexose and amino acid cotransport on high-salt intake to amiloride-sensitive sodium channel expression on low-salt (LS) diets. The present experiments were designed to investigate the role of aldosterone in suppression of the colonic sodium-glucose luminal cotransporter (SGLT). LS-adapted hens were resalinated with or without simultaneous aldosterone treatment. Changes in the electrophysiological responses and SGLT protein expression levels were examined at 1, 3, and 7 days of treatment. Serum aldosterone levels fell from ∼400 pmol/l in LS-adapted hens to values below the detection limit (<44 pmol/l) after 1 day of resalination. At the same time, glucose-stimulated short circuit current ( I SC) increased from 20.9 ± 8.7 to 56.3 ± 15.5 μA/cm2, whereas amiloride-sensitive I SC decreased from −68.9 ± 12.7 μA/cm2 on LS to +0.6 ± 12.0 μA/cm2. Glucose-stimulated I SC increased further at 3 and 7 days of resalination, whereas amiloride-sensitive I SC remained suppressed. When resalinated birds were simultaneously treated with aldosterone, the LS pattern of high amiloride-sensitive I SC and low glucose-stimulated I SC was maintained. Immunoblotting results from the same tissues demonstrated that SGLT-like protein expression increased following resalination. Aldosterone treatment completely blocked this effect. These results demonstrate that aldosterone suppresses both activity and protein expression of hen colonic SGLT. Resalination either through decreased aldosterone or other factors may be able to activate SGLT activity independently of increases in protein expression.