Effect of hypercapnic hypoxia and bacterial infection (Vibrio campbellii) on protein synthesis rates in the Pacific whiteleg shrimp,Litopenaeus vannamei

Abstract

Estuarine species frequently encounter areas of simultaneously low dissolved O2(hypoxia) and high CO2(hypercapnia). Organisms exposed to hypoxia experience a metabolic depression that serves to decrease ATP utilization and O2demand during stress. This downregulation is typically facilitated by a reduction in protein synthesis, a process that can be responsible for up to 60% of basal metabolism. The added effects of hypercapnia, however, are unclear. Certain decapods also exhibit a metabolic depression in response to bacterial challenges, leading us to hypothesize that protein synthesis may also be reduced during infection. In the present study, we examined the effects of hypoxia (H), hypercapnic hypoxia (HH), and bacterial infection ( Vibrio campbellii) on tissue-specific (muscle and hepatopancreas) fractional protein synthesis rates ( ks) in Litopenaeus vannamei. We observed a significant decrease in ksin muscle after 24 h exposure to both H and HH, and in hepatopancreas after 24 h exposure to HH. Thus ksis responsive to changes in O2, and the combined effect of hypercapnic hypoxia on ksis more severe than hypoxia alone. These reductions in ksappear to be driven by changes in RNA translational efficiency ( kRNA), and not RNA capacity ( Cs). Bacterial infection, however, had no significant effect on ksin either tissue. These results suggest that crustaceans reduce metabolic demand during environmental hypoxia by reducing global protein synthesis, and that this effect is magnified when hypercapnia is concomitantly present. Conversely, an immune-mediated metabolic depression is not associated with a decrease in overall protein production.