Chemoreceptor-sensitive neurons in commissural subnucleus of nucleus tractus solitarius of the rat

Abstract

Experiments were carried out in pentobarbital-anesthetized, vagotomized, paralyzed, and artificially ventilated male Wistar rats with a pneumothorax. Blood pressure, heart rate, and phrenic nerve activity were recorded. Extracellular neuronal recordings were made by glass micropipettes in a midline area in the commissural subnucleus of the nucleus tractus solitarius. At the end of the experiment, the recording sites were marked. Chemoreceptors were stimulated by tracheal administration of N2 or injection of CO2-saturated saline (50 microliters) near the carotid body. The baroreceptors were stimulated by an intravenous injection of phenylephrine (1-3 micrograms). Neurons that were excited by chemoreceptor stimulation but not baroreceptor stimulation were found in the commissural subnucleus of nucleus tractus solitarius and were designated as chemoreceptor-sensitive neurons. They exhibited a continuous discharge (9.9 +/- 1.3 spikes/s) that showed no apparent relation to either phrenic nerve bursts or arterial pulses. Such neurons were not found in the rostral portions of the nucleus tractus solitarius. These results confirm and extend our previous reports in which a discrete chemoreceptor projection site was identified in the commissural subnucleus of the rat.