Noradrenergic and GABAergic systems in the medial hypothalamus are activated during hypoglycemia

Abstract

Noradrenergic and GABAergic systems in the medial hypothalamus influence plasma glucose and may be activated during glucoprivation. Microdialysis probes were placed into the ventromedial nucleus (VMH), lateral hypothalamus (LHA), and paraventricular nucleus (PVH) of male Sprague-Dawley rats to monitor extracellular concentrations of norepinephrine (NE) and GABA. During systemic hypoglycemia, induced by insulin (1.0 U/kg), NE concentrations increased in the VMH ( P < 0.05) and PVH ( P = 0.06) in a bimodal fashion during the first 10 min and 20–30 min after insulin administration. In the VMH, GABA concentrations increased ( P < 0.05) in a similar manner as NE. Extracellular NE concentrations in the LHA were slightly lower ( P = 0.13), and GABA levels remained at baseline. The increases in NE and GABA in the VMH were absent during euglycemic clamp; however, NE in the PVH still increased, reflecting a direct response to hyperinsulinemia. On the basis of these data, we propose that the activity of noradrenergic afferents to the medial hypothalamus is increased during hypoglycemia and influences the activity of local GABAergic systems to activate appropriate physiological compensatory mechanisms.