Affiliation:
1. Department of Physiological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, United Kingdom; and Max-Planck-Institut für molekulare Physiologie, 44227 Dortmund, Germany
Abstract
Membrane transport systems for Pitransport are key elements in maintaining homeostasis of Piin organisms as diverse as bacteria and human. Two Na-Picotransporter families with well-described functional properties in vertebrates, namely NaPi-II and NaPi-III, show conserved structural features with prokaryotic origin. A clear vertical relationship can be established among the mammalian protein family NaPi-III, a homologous system in C. elegans, the yeast system Pho89, and the bacterial Pitransporter Pit. An alternative lineage connects the mammalian NaPi-II-related transporters with homologous proteins from Caenorhabditis elegans and Vibrio cholerae. The present review focuses on the molecular evolution of the NaPi-II protein family. Preliminary results indicate that the NaPi-II homologue cloned from V. cholerae is indeed a functional Pitransporter when expressed in Xenopus oocytes. The closely related NaPi-II isoforms NaPi-IIa and NaPi-IIb are responsible for regulated epithelial Na-dependent Pitransport in all vertebrates. Most species express two different NaPi-II proteins with the exception of the flounder and Xenopus laevis, which rely on only a single isoform. Using an RT-PCR-based approach with degenerate primers, we were able to identify NaPi-II-related mRNAs in a variety of vertebrates from different families. We hypothesize that the original NaPi-IIb-related gene was duplicated early in vertebrate development. The appearance of NaPi-IIa correlates with the development of the mammalian nephron.
Publisher
American Physiological Society
Subject
Physiology (medical),Physiology
Cited by
131 articles.
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