A method for measuring deviations from equilibrium of the glucose anomers in blood

Abstract

A method for detecting deviations from equilibrium values of β-d-glucose has been developed. This method is dependent upon the specificity of glucose-oxidase for β-d-glucose and the catalytic effect of hydroxyl ions on mutarotation. The method has been used to monitor the blood stream of anesthetized normal dogs and dogs given epinephrine, glucagon, or insulin. It has also been used with alloxan-diabetic dogs. Since the method will detect deviations from equilibrium when agr- or β-d-glucose infusions are given and since in the above situations no impressive deviations were seen, it has been concluded that in the anesthetized dog the glucose is at or very near equilibrium and that glucagon and epinephrine do not alter the equilibrium. monitoring blood glucose; epinephrine on glucose anomers; glucagon on glucose anomers; insulin on glucose anomers Submitted on August 24, 1964