Reduced surface pH and upregulated AE2 anion exchange in SLC26A3-deleted polarized intestinal epithelial cells-Reference-Cited by-同舟云学术

Reduced surface pH and upregulated AE2 anion exchange in SLC26A3-deleted polarized intestinal epithelial cells

Published:2024-03-01 Issue:3 Volume:326 Page:C829-C842
ISSN:0363-6143
Container-title:American Journal of Physiology-Cell Physiology
language:en
Short-container-title:American Journal of Physiology-Cell Physiology

Author:

Amiri Mahdi¹^ORCID,Jiang Min¹,Salari Azam¹,Xiu Renjie¹,Alper Seth L.²³^ORCID,Seidler Ursula E.¹^ORCID

Affiliation:

1. Department of Gastroenterology, Hannover Medical School, Hannover, Germany

2. Division of Nephrology, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States

3. Department of Medicine, Harvard Medical School, Boston, Massachusetts, United States

Abstract

SLC26A3 gene mutations cause chloride-losing diarrhea. To understand how colonic enterocytes adapt, SLC26A3 was deleted in Caco2BBe cells using CRISPR/Cas9. In comparison to the wild-type cells, SLC26A3 knockout cells showed similar growth and transepithelial resistance but substantially reduced apical Cl−/HCO3− exchange rates, and an acidic surface pH. Steady-state intracellular pH was comparable between the WT and KO cells due to increased basolateral AE2 expression and function.

Funder

Deutsche Forschungsgemeinschaft

Publisher

American Physiological Society

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpcell.00590.2023

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