Tbxt alleviates senescence and apoptosis of nucleus pulposus cells through Atg7-mediated autophagy activation during intervertebral disk degeneration-Reference-Cited by-同舟云学术

Tbxt alleviates senescence and apoptosis of nucleus pulposus cells through Atg7-mediated autophagy activation during intervertebral disk degeneration

Published:2024-08-01 Issue:2 Volume:327 Page:C237-C253
ISSN:0363-6143
Container-title:American Journal of Physiology-Cell Physiology
language:en
Short-container-title:American Journal of Physiology-Cell Physiology

Author:

Yue Caichun¹²,Wu Yinghui¹²,Xia Yanzhang¹²,Xin Tianwen¹²,Gong Yuhao¹²,Tao Linfeng³,Shen Cong⁴,Zhu Yue⁵,Shen Minghong⁶,Wang Donglai¹²,Shen Jun¹²^ORCID

Affiliation:

1. Department of Orthopedics Surgery, Suzhou Municipal Hospital/The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, People’s Republic of China

2. Suzhou Key Laboratory of Orthopedic Medical Engineering, Suzhou Municipal Hospital/The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, People’s Republic of China

3. Department of Critical Care Medicine, Suzhou Municipal Hospital/The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, People’s Republic of China

4. State Key Laboratory of Reproductive Medicine, Center for Reproduction and Genetics, Suzhou Municipal Hospital/The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, People’s Republic of China

5. Department of Breast and Thyroid Surgery, Suzhou Municipal Hospital/The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, People’s Republic of China

6. Department of Pathology, Suzhou Municipal Hospital/The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, People’s Republic of China

Abstract

This study investigates the role of Tbxt in IDD. The results demonstrate that knockdown of Tbxt exacerbates H2O2-induced senescence and apoptosis in NPCs and IDD, whereas upregulation of Tbxt significantly protects against IDD both in vivo and in vitro. Mechanistically, in the nucleus, Tbxt enhances the transcription of ATG7, leading to increased expression of ATG7 protein levels. This, in turn, promotes elevated autophagy levels, ultimately alleviating IDD.

Funder

Key Project of Social Development in Jiangsu-Clinical Frontier Technology

Suzhou Gusu Medical Youth Talent

Technology Project of Suzhou Health Commission

Publisher

American Physiological Society

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpcell.00126.2024

Reference50 articles.

1. Epidemiological features of chronic low-back pain

2. Low back pain

3. Herniated Lumbar Intervertebral Disk

4. Low back pain

5. What low back pain is and why we need to pay attention