CRISPR/Cas9-mediated deletion of adipocyte genes associated with NAFLD alters adipocyte lipid handling and reduces steatosis in hepatocytes in vitro-Reference-Cited by-同舟云学术

CRISPR/Cas9-mediated deletion of adipocyte genes associated with NAFLD alters adipocyte lipid handling and reduces steatosis in hepatocytes in vitro

Published:2023-11-01 Issue:5 Volume:325 Page:C1178-C1189
ISSN:0363-6143
Container-title:American Journal of Physiology-Cell Physiology
language:en
Short-container-title:American Journal of Physiology-Cell Physiology

Author:

Lopez-Yus Marta¹²³,Frendo-Cumbo Scott⁴,del Moral-Bergos Raquel¹²³,Garcia-Sobreviela Maria Pilar¹³,Bernal-Monterde Vanesa¹³⁵,Rydén Mikael⁴,Lorente-Cebrian Silvia¹³⁶,Arbones-Mainar Jose M.¹²³⁷^ORCID

Affiliation:

1. Adipocyte and Fat Biology Laboratory (AdipoFat), Translational Research Unit, University Hospital Miguel Servet, Zaragoza, Spain

2. Instituto Aragonés de Ciencias de la Salud (IACS), Zaragoza, Spain

3. Instituto de Investigación Sanitaria (IIS) Aragón, Zaragoza, Spain

4. Department of Medicine (H7), Karolinska Institutet, Karolinska University Hospital Huddinge, Huddinge, Sweden

5. Gastroenterology Department, Miguel Servet University Hospital, Zaragoza, Spain

6. Departamento de Farmacología, Fisiología y Medicina Legal y Forense, Universidad de Zaragoza, Instituto Agroalimentario de Aragón (IA2) (Universidad de Zaragoza-CITA), Zaragoza, Spain

7. CIBER Fisiopatología Obesidad y Nutrición (CIBERObn), Instituto Salud Carlos III, Madrid, Spain

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology successfully edited genomic DNA of human adipose-derived mesenchymal stem cells (hADMSC). SOCS3, SIK1, and DUSP1 regulate adipocyte lipid handling. Silencing SOCS3, SIK1, and DUSP1 expression in hADMSC-derived adipocytes reduces hepatocyte lipid storage in vitro.

Funder

Spanish Ministry of Science

EC | European Research Council

European Molecular Biology Organization

Gobierno de Aragón

KI | Center for Innovative Medicine

Karolinska Institutet

Knut och Alice Wallenbergs Stiftelse

MEC | Instituto de Salud Carlos III

Novo Nordisk Foundation Center for Basic Metabolic Research

Stockholm läns landsting

Stockholms Läns Landsting