Stretch response of the mechano-gated channel TMEM63A in membrane patches and single cells-Reference-Cited by-同舟云学术

Stretch response of the mechano-gated channel TMEM63A in membrane patches and single cells

Published:2024-02-01 Issue:2 Volume:326 Page:C622-C631
ISSN:0363-6143
Container-title:American Journal of Physiology-Cell Physiology
language:en
Short-container-title:American Journal of Physiology-Cell Physiology

Author:

Niloy Sayeman Islam¹,Strege Peter R.¹²^ORCID,Hannan Elizabeth C.¹,Cowan Luke M.¹^ORCID,Linsenmeier Fabian³,Friedrich Oliver³,Farrugia Gianrico¹²^ORCID,Beyder Arthur¹²^ORCID

Affiliation:

1. Enteric Neuroscience Program (ENSP), Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota, United States

2. Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, United States

3. Institute of Medical Biotechnology, Department of Chemical and Biological Engineering, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany

Abstract

TMEM63A has biophysical features distinctive for mechano-gated cation channels, but some properties are less clear, including no functional information in whole cells. We report that pressure-dependent yet voltage-independent TMEM63A currents in cell membrane patches correlated with cell size. In addition, radial stretch of whole cells on flexible membranes increased the [Ca2+]i responses more in TMEM63A-transfected cells. Inward TMEM63A currents in response to high stretch can depolarize plasma membranes and contribute to a sustained [Ca2+]i increase.

Funder

HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases

Publisher

American Physiological Society

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpcell.00583.2023

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