G-protein tonic inhibition of calcium channels in pancreatic β-cells-Reference-Cited by-同舟云学术

G-protein tonic inhibition of calcium channels in pancreatic β-cells

Published:2023-09-01 Issue:3 Volume:325 Page:C592-C598
ISSN:0363-6143
Container-title:American Journal of Physiology-Cell Physiology
language:en
Short-container-title:American Journal of Physiology-Cell Physiology

Author:

Quiroz-Acosta Tayde¹,Bermeo Karina¹^ORCID,Arenas Isabel¹,Garcia David E.¹^ORCID

Affiliation:

1. Department of Physiology, Faculty of Medicine, Universidad Nacional Autónoma de México, UNAM, Ciudad de México, Mexico

Abstract

Our results support the hypothesis that GPCRs tonically inhibit HVA Ca2+ channels in pancreatic β-cells. A voltage-clamp protocol with a strong depolarizing prepulse was used to unmask voltage-dependent inhibition of Ca2+ channels. The novelty of these results strengthens the critical role of Gβγ’s in Ca2+ channel regulation, highlighting kinetic slowing and increased facilitation ratio. Together, HVA Ca2+ channels of pancreatic β-cells are constitutively inhibited by GPCRs underlying fine-tuning modulation of insulin secretion.

Funder

UNAM | Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México

Alexander von Humboldt Stiftung

Publisher

American Physiological Society

Subject

Cell Biology,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpcell.00447.2022

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