Nonpolarized surface distribution and delivery of human CD7 in polarized MDCK cells

Abstract

Madin-Darby canine kidney (MDCK) cells grown on permeable supports have served as the most common experimental system for in vitro studies of the generation and maintenance of epithelial surface polarity. Protein targeting to the apical and basolateral plasmalemmal domains of these and other polarized epithelia has been suggested to rely on targeting sequences. Two simple sorting models for MDCK cells have proposed active sorting to a single domain, with "default" movement to the other domain. Examples of both apical and basal sorting signals have been found to support each hypothesis, but the idea of a default pathway has remained in question. Indeed, all endogenous and heterologous wild-type proteins so far studied in MDCK cells achieve polarized distributions at steady state. It is not known whether these selected proteins are representative of all surface membrane proteins or represent only a subset. We report here the apparent absence of sorting by MDCK cells of the transmembrane protein of T-cells, CD7. CD7 is expressed at similar density in apical and basolateral membranes of MDCK cells as assessed by both immunocytological and biochemical criteria. Furthermore, CD7 appears to be directly sorted to both surfaces at similar rates and turns over at both surfaces at similar rates. The nonpolarized distribution of CD7 appears independent of its level of expression. CD7 may identify a "bulk-flow" default pathway for plasma membrane proteins expressed in polarized MDCK cells.