Electrophysiological properties of human adipose tissue-derived stem cells

Abstract

Human adipose tissue-derived stem cells (hASCs) represent a potentially valuable cell source for clinical therapeutic applications. The present study was designed to investigate properties of ionic channel currents present in undifferentiated hASCs and their impact on hASCs proliferation. The functional ion channels in hASCs were analyzed by whole-cell patch-clamp recording and their mRNA expression levels detected by RT-PCR. Four types of ion channels were found to be present in hASCs: most of the hASCs (73%) showed a delayed rectifier-like K+ current ( IKDR); Ca2+-activated K+ current ( IKCa) was detected in examined cells; a transient outward K+ current ( Ito) was recorded in 19% of the cells; a small percentage of cells (8%) displayed a TTX-sensitive transient inward sodium current ( INa.TTX). RT-PCR results confirmed the presence of ion channels at the mRNA level: Kv1.1, Kv2.1, Kv1.5, Kv7.3, Kv11.1, and hEAG1, possibly encoding IKDR; MaxiK, KCNN3, and KCNN4 for IKCa; Kv1.4, Kv4.1, Kv4.2, and Kv4.3 for Ito and hNE-Na for INa.TTX. The IKDR was inhibited by tetraethyl ammonium (TEA) and 4-aminopyridine (4-AP), which significantly reduced the proliferation of hASCs in a dose-dependent manner ( P < 0.05), as suggested by bromodeoxyurindine (BrdU) incorporation. Other selective potassium channel blockers, including linopiridine, iberiotoxin, clotrimazole, and apamin also significantly inhibited IKDR. TTX completely abolished INa.TTX. This study demonstrates for the first time that multiple functional ion channel currents such as IKDR, IKCa, Ito, and INa.TTX are present in undifferentiated hASCs and their potential physiological function in these cells as a basic understanding for future in vitro experiments and in vivo clinical investigations.