Author:
Akel Ahmad,Wagner Carsten A.,Kovacikova Jana,Kasinathan Ravi. S.,Kiedaisch Valentin,Koka Saisudha,Alper Seth L.,Bernhardt Ingolf,Wieder Thomas,Huber Stephan M.,Lang Florian
Abstract
Genetic defects of anion exchanger 1 (AE1) may lead to spherocytic erythrocyte morphology, severe hemolytic anemia, and/or cation leak. In normal erythrocytes, osmotic shock, Cl−removal, and energy depletion activate Ca2+-permeable cation channels with Ca2+-induced suicidal erythrocyte death, i.e., surface exposure of phosphatidylserine, cell shrinkage, and membrane blebbing, all features typical for apoptosis of nucleated cells. The present experiments explored whether AE1 deficiency favors suicidal erythrocyte death. Peripheral blood erythrocyte numbers were significantly smaller in gene-targeted mice lacking AE1 ( AE1−/−mice) than in their wild-type littermates ( AE1+/+mice) despite increased percentages of reticulocytes ( AE1−/−: 49%, AE1+/+: 2%), an indicator of enhanced erythropoiesis. Annexin binding, reflecting phosphatidylserine exposure, was significantly larger in AE1−/−erythrocytes/reticulocytes (∼10%) than in AE1+/+erythrocytes (∼1%). Osmotic shock (addition of 400 mM sucrose), Cl−removal (replacement with gluconate), or energy depletion (removal of glucose) led to significantly stronger annexin binding in AE1−/−erythrocytes/reticulocytes than in AE1+/+erythrocytes. The increase of annexin binding following exposure to the Ca2+ionophore ionomycin (1 μM) was, however, similar in AE1−/−and in AE1+/+erythrocytes. Fluo3 fluorescence revealed markedly increased cytosolic Ca2+permeability in AE1−/−erythrocytes/reticulocytes. Clearance of carboxyfluorescein diacetate succinimidyl ester-labeled erythrocytes/reticulocytes from circulating blood was more rapid in AE1−/−mice than in AE1+/+mice and was accelerated by ionomycin treatment in both genotypes. In conclusion, lack of AE1 is associated with enhanced Ca2+entry and subsequent scrambling of cell membrane phospholipids.
Publisher
American Physiological Society
Cited by
24 articles.
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