Abstract
The study of muscle physiology has undergone many changes over the past 25 years and has moved from purely physiological studies to those intimately intertwined with molecular and cell biological questions. To ask these questions, it is necessary to be able to transfer genetic reagents to cells both in culture and, ultimately, in living animals. Over the past 10 years, a number of different chemical and physical approaches have been developed to transfect living skeletal, smooth, and cardiac muscle systems with varying success and efficiency. This review provides a survey of these methods and describes some more recent developments in the field of in vivo gene transfer to these various muscle types. Both gene delivery for overexpression of desired gene products and delivery of nucleic acids for downregulation of specific genes and their products are discussed to aid the physiologist, cell biologist, and molecular biologist in their studies on whole animal biology.
Publisher
American Physiological Society
Reference134 articles.
1. Acsadi G, Jiao SS, Jani A, Duke D, Williams P, Chong W, and Wolff JA. Direct gene transfer and expression into rat heart in vivo. New Biol 3: 71–81, 1991.
2. Gene transfer into muscle by electroporation in vivo
3. Andre F and Mir LM. DNA electrotransfer: its principles and an updated review of its therapeutic applications. Gene Ther 11, Suppl 1: S33–S42, 2004.
4. In VivoTransfer Efficiency of Antisense Oligonucleotides into the Myocardium Using HVJ–Liposome Method
5. Optimization of Nonviral Gene Transfer of Vascular Smooth Muscle Cells in Vitro and in Vivo
Cited by
59 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献